![Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit](https://adipogen.com/pub/media/catalog/product/s/b/sbb-kf0120_signal-to-background-web_1.png)
Cullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit
![Research Use Only](static/images/certificates/ruo.jpg)
SBB-KF0120
Product group Chemicals
Overview
- SupplierSouth Bay Bio
- Product NameCullin4a/Rbx1/DDB1/CRBN E3 Ligase Complex TR-FRET Kit
- Delivery Days Customer10
- CertificationResearch Use Only
- Scientific DescriptionSouth Bay Bios Cullin4a/Rbx1/DDB1/CRBN Neddylated E3 Ligase TR-FRET Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto the E3 complex in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto CRBN (or substrates) in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate true signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.
- Storage Instruction-20°C,-80°C
- UNSPSC12352200