Western blot using Rocklands affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1microg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
KIAA0652 (ATG13) Rabbit Polyclonal Antibody
TA397436S
Overview
- SupplierOriGene
- Product NameKIAA0652 (ATG13) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID9776
- Target nameATG13
- Target descriptionautophagy related 13
- Target synonymsATG13 autophagy related 13 homolog; autophagy-related protein 13; KIAA0652; PARATARG8
- HostRabbit
- Scientific DescriptionATG13 Antibody
- ReactivityHuman
- UNSPSC12352203