CXCL4 / PF4 Antibody (Biotin)

LifeSpan BioSciences

CXCL4 / PF4 Antibody (Biotin)

23

Human PF-4

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hPF-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PF-4 (LS-C104447) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.25 - 1 µg/ml), WB (0.1 - 0.2 µg/ml) The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hPF-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PF-4 (LS-C104447) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA

Research Use Only

Polyclonal

Biotin

...

PF4

platelet factor 4

chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; iroplact; oncostatin-A; PF-4; platelet factor 4; SCYB4

Rabbit

Human

-20°C,2°C to 8°C,RT

12352203