Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_T-44942_20230707_WB_M_spleen_23071223_941.webp "Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_T-44942_20230728_IHC-P_1_23080901_231.webp "CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded Raji xenograft. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_T-44942_20230728_IHC-P_23080901_299.webp "CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded Raji xenograft. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_T-44942_20230728_IHC-P_R_23080901_998.webp "CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_45159_20230908_WB_Fraction_23091319_457.webp "Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_45159_20230915_WB_shRNA_watermark_23091901_973.webp "Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_45159_20231110_ICC_IF_B_23111422_223.webp "CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded Raji xenograft. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:200 and competitor's antibody (MAB42273) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_45159_20240301_IHC-P_competitor_24030600_600.webp "CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded Raji xenograft. CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:200 and competitor's antibody (MAB42273) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.")
![CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638193/GTX638193_45159_20241122_ICC_IF_24120522_412.webp "CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).")
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CXCR7 antibody [HL2189]
GTX638193
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACKR3
Overview
- SupplierGeneTex
- Product NameCXCR7 antibody [HL2189]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2189
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID57007
- Target nameACKR3
- Target descriptionatypical chemokine receptor 3
- Target synonymsCMKOR1, CXC-R7, CXCR-7, CXCR7, GPR159, RDC-1, RDC1, atypical chemokine receptor 3, C-X-C chemokine receptor type 7, G protein-coupled receptor, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, chemokine (C-X-C motif) receptor 7, chemokine orphan receptor 1
- HostRabbit
- IsotypeIgG
- Protein IDP25106
- Protein NameAtypical chemokine receptor 3
- Scientific DescriptionThis gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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