Cyanine 5.5 hydrazide

Antibodies.com

Cyanine 5.5 hydrazide

7

Research Use Only

95% (by 1H NMR and HPLC-MS).

C40H46Cl2N4O

669.73 kDa

Cyanine 5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides quickly react with carbonyl compounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates. Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium periodate and labeled with Cyanine 5.5 hydrazide to form Cyanine 5.5 labeled antibodies. Since sugar residues in antibodies are located far from variable, epitope-binding site, this method is particularly good for the labeling of antibodies. Cyanine 5.5 is a near infrared fluorophore, an analog of Cy5.5®. It is particularly suitable for the applications which require low fluorescence background. The absorption and emission wavelength of the dye exhibit significant tissue penetration, so Cyanine 5.5 is suitable for in vivo NIR imaging applications.

Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.

12352200