Western blot using GeneTexs affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).
Western blot using GeneTexs affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).
Cyclin E2 antibody
GTX48724
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCcne2
Overview
- SupplierGeneTex
- Product NameCyclin E2 antibody
- Delivery Days Customer9
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.17 mg/ml
- ConjugateUnconjugated
- Gene ID12448
- Target nameCcne2
- Target descriptioncyclin E2
- Target synonymsG1/S-specific cyclin-E2
- HostRabbit
- IsotypeIgG
- Scientific DescriptionCyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions.Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.
- ReactivityHuman, Mouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Targeting DNA and mutant p53 by a naphthalimide derivative, NA20, exhibits selective inhibition in gastric tumorigenesis by blocking mutant p53-EGFR signaling pathway. Zhang G et al., 2020 Nov 15, Eur J PharmacolRead more