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ProductsBack/CYLD Antibody (Phospho-Ser418) (OABF01160)

CYLD Antibody (Phospho-Ser418) (OABF01160)

OABF01160
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCYLD
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CYLD Antibody (Phospho-Ser418) (OABF01160)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Format
    Liquid Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
  • Gene ID1540
  • Target name
    CYLD
  • Target description
    CYLD lysine 63 deubiquitinase
  • Target synonyms
    BRSS; CDMT; CYLD1; CYLDI; cylindromatosis (turban tumor syndrome); deubiquitinating enzyme CYLD; EAC; FTDALS8; MFT; MFT1; probable ubiquitin carboxyl-terminal hydrolase CYLD; SBS; TEM; ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD; USPL2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYLD using anti-CYLD antibody (PB9543). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYLD antigen affinity purified polyclonal antibody (Catalog # PB9543) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYLD at approximately 107 kDa. The expected band size for CYLD is at 107 kDa.
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