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ProductsBack/CYP4F2 Antibody - N-terminal region (OASG02010)

CYP4F2 Antibody - N-terminal region (OASG02010)

OASG02010
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCYP4F2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CYP4F2 Antibody - N-terminal region (OASG02010)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID8529
  • Target name
    CYP4F2
  • Target description
    cytochrome P450 family 4 subfamily F member 2
  • Target synonyms
    CPF2, cytochrome P450 4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, CYPIVF2, arachidonic acid omega-hydroxylase, cytochrome P450, family 4, subfamily F, polypeptide 2, cytochrome P450, subfamily IVF, polypeptide 2, cytochrome P450-LTB-omega, docosahexaenoic acid omega-hydroxylase, leukotriene-B(4) 20-monooxygenase 1, leukotriene-B(4) omega-hydroxylase 1, phylloquinone omega-hydroxylase CYP4F2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP4F2 using anti-CYP4F2 antibody (A01737-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP4F2 antigen affinity purified polyclonal antibody (A01737-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP4F2 at approximately 50 kDa. The expected band size for CYP4F2 is at 60 kDa.
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