Cytochrome C (Mitochondrial Marker)(7H8.2C12 + CYCS/1010), CF405S conjugate, 0.1mg/mL [26628-22-8]

Biotium

Cytochrome C (Mitochondrial Marker)(7H8.2C12 + CYCS/1010), CF405S conjugate, 0.1mg/mL

9

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

7H8.2C12 CYCS/1010

0.1 mg/ml

Other Conjugate

CYCS

cytochrome c, somatic

CYC; cytochrome c; HCS; THC4

Mouse

IgG2b

Cytochrome c

Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Animal

Bovine, Human, Mouse, Rat

2°C to 8°C

12352203