Cytokeratin 18 Antibody Cocktail

Biorbyt

Cytokeratin 18 Antibody Cocktail

16

Optimal dilution of the Cytokeratin 18 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Flow Cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2) FACS, IHC-P, WB

Research Use Only

Monoclonal

Clone Cocktail

Unconjugated

KRT18

keratin 18

cell proliferation-inducing gene 46 protein; CK-18; CYK18; cytokeratin 18; K18; keratin 18, type I; keratin, type I cytoskeletal 18

Mouse

IgG1

Keratin, type I cytoskeletal 18

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Human

-20°C,2°C to 8°C

12352203