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ProductsBack/Cytokeratin 20 (CK 20) Polyclonal Antibody

Cytokeratin 20 (CK 20) Polyclonal Antibody

CAU26290
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetKrt20
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cytokeratin 20 (CK 20) Polyclonal A
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID66809
  • Target name
    Krt20
  • Target description
    keratin 20
  • Target synonyms
    9030623C06Rik; Ck20; Ck-20; cytokeratin 20; K20; keratin, type I cytoskeletal 20; Krt21
  • Host
    Rabbit
  • Protein IDQ9D312
  • Protein Name
    Keratin, type I cytoskeletal 20
  • Scientific Description
    The Cytokeratin 20 (CK 20) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 5. Flow Cytometry analysis of CACO-2 cells using anti-Krt20 antibody (A02828-2). Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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