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(Cyto)toxicity

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HEK 293 cells were seeded at 20000 cells per well and allowed to grow for 24 hours. After adding various concentrations of Triton X-100, LDH Cytotoxicity Assay Reagent was added. Cells were incubated for 30 minutes at 37°C and 5% CO2, and then read on a colorimetric plate reader.
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Arigo Biolaboratories
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