D-Lactate Assay Kit (Fluorometric)

LifeSpan BioSciences

D-Lactate Assay Kit (Fluorometric)

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For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Spectrophotometry (fluorometric) For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

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Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. This lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the lactate concentration in the sample.

-20°C

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