DBCO-PEG4-Desthiobiotin, Dibenzylcyclooctyne-PEG4-Desthiobiotin

Jena Bioscience

DBCO-PEG4-Desthiobiotin, Dibenzylcyclooctyne-PEG4-Desthiobiotin

5

Research Use Only

>95%

C39H53N5O8

719.87 g/mol

DBCO-PEG4-Desthiobiotin is suitable for the introduction of a desthiobiotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction. The hydrophilic PEG4 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the desthiobiotin moiety and thus the detection efficiency of the desthiobiotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose. Desthiobiotin binds less tightly to streptavidin (KD = 10-11 M) than Biotin (KD = 10-15M). Desthiobiotinylated molecules are therefore easily eluted from the complex in the presence of excess Biotin.

C1(=O)N[C@H]([C@H](N1)CCCCCC(=O)NCCOCCOCCOCCOCCC(=O)NCCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)C

-20°C

12352200