Western blot showing detection of 0.1 microg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 microm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Western blot showing detection of 0.1 microg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 microm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Dbp-B antibody
GTX48802
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBacteria
TargetdbpB
Overview
- SupplierGeneTex
- Product NameDbp-B antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1194341
- Target namedbpB
- Target descriptiondecorin-binding protein DbpB
- Target synonymsBB_A25; BB_RS05220; decorin-binding protein DbpB
- HostRabbit
- IsotypeIgG
- ReactivityBacteria
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203