DDDDK tag antibody [FG4R]

GeneTex

DDDDK tag antibody [FG4R]

9

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Recommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000-3000 Immunostaining: Use at a dilution of 1:500 - 1:2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

FG4R

1 mg/ml

Unconjugated

Mouse

IgG1

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Other Species

-20°C or -80°C,2°C to 8°C

12352203