Effect of Smac/Diablo Peptide (54-64), Biotin on XIAP binding to Diablo.
Effect of Smac/Diablo Peptide (54-64), Biotin on XIAP binding to Diablo.
Diablo:XIAP Inhibitor Screening Assay Kit
82516
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameDiablo:XIAP Inhibitor Screening Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe Diablo:XIAP Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between Diablo (also named Smac) and E3 ubiquitin-protein ligase XIAP (X-linked inhibitor of apoptosis, also known as mammalian IAP homolog A) for screening and profiling applications. The Diablo:XIAP Inhibitor Screening Assay Kit comes with enough purified Diablo (amino acids 56-239) and XIAP proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with purified Diablo protein. After coating and blocking, XIAP is added in an optimized assay buffer. Next, unbound XIAP is washed away and the plate is incubated with a primary antibody, followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the binding of XIAP to Diablo.
- Storage InstructionSee manual
- UNSPSC41116133