Direct qPCR ProbesMaster, Robust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix

Jena Bioscience

Direct qPCR ProbesMaster, Robust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix

5

Research Use Only

Direct qPCR ProbesMaster is designed for quantitative real-time analysis of target DNA directly from blood, swabs and animal- or plant tissue. The mix allows robust amplification avoiding the requirement of any prior DNA purification procedures. The mix is recommended for use with dual-labeled fluorescent probes, e.g. TaqMan®, Molecular Beacon or Scorpion probes. It provides a powerful tool for multiplex-quantification of sample DNA in a broad dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct detection of viral or bacterial DNA in nasal or throat swabsDirect PCR from blood samplesDirect amplification of target DNA from various tissue samplesPoint-of-Care diagnostics. Multiplexing Capability Real-time PCR technology based on dual-labeled DNA probes provides a high sensitive and specific PCR system with multiplexing capability. The simultaneous detection of multiple targets in a single tube requires a primer/probe set for each target amplification. Sequences and concentrations of primers and probes should be optimized to avoid mutual influence, secondary structures and primer-dimer formations. Amplification of each target is detected in a separate fluorescence channel. ROX Reference Dye The mix can also be used in combination with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.

2°C to 8°C,-20°C

41116133