Identification of Dis2 phosphorylated at T316 by western blotting with the antibody. S. pombe crude extracts prepared from mitotic and G2-phase were analysed by WB. Dis2 and phosphorylated Dis2 were detected with anti-Dis2 antibody and this antibody (GTX64105), respectively. Phosphorylation is increased in mitosis and sensitive to lambda-phosphatase. The antibody was used at 1/1,000 dilution in PBS containing 0.1% Tween and 1% milk. Courtesy of Mr M. Swaffer at Cancer Research UK
Identification of Dis2 phosphorylated at T316 by western blotting with the antibody. S. pombe crude extracts prepared from mitotic and G2-phase were analysed by WB. Dis2 and phosphorylated Dis2 were detected with anti-Dis2 antibody and this antibody (GTX64105), respectively. Phosphorylation is increased in mitosis and sensitive to lambda-phosphatase. The antibody was used at 1/1,000 dilution in PBS containing 0.1% Tween and 1% milk. Courtesy of Mr M. Swaffer at Cancer Research UK
Dis2 (phospho Thr316) (S. pombe) antibody
GTX64105
Overview
- SupplierGeneTex
- Product NameDis2 (phospho Thr316) (S. pombe) antibody
- Delivery Days Customer9
- Antibody SpecificityReacts with S. pombe Dis2 protein phosphorylated at Thr316. Not tested with other species
- Application Supplier NoteWestern blotting (1:1,000-1:2,000 dilution).
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID2541193
- Target namedis2
- Target descriptionserine/threonine protein phosphatase PP1
- Target synonymsbws1; sds1; serine/threonine protein phosphatase PP1; SPBC776.02c
- HostRabbit
- IsotypeIgG
- Protein IDP13681
- Protein NameSerine/threonine-protein phosphatase PP1-1
- ReactivityYeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203