DNA ligase IV antibody
GTX108820
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLIG4
Overview
- SupplierGeneTex
- Product NameDNA ligase IV antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3981
- Target nameLIG4
- Target descriptionDNA ligase 4
- Target synonymsDNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; LIG4S; ligase IV, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThe protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation. Chen SH et al., 2019 Feb 20, Nucleic Acids ResRead more
- Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes. Chakraborty A et al., 2016 Oct 5, Nat CommunRead more
- A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy. Bee L et al., 2015 Jul, EMBO Mol MedRead more
- c-MYC Generates Repair Errors via Increased Transcription of Alternative-NHEJ Factors, LIG3 and PARP1, in Tyrosine Kinase-Activated Leukemias. Muvarak N et al., 2015 Apr, Mol Cancer ResRead more