ICC/IF analysis of mouse embryonic stem cells using GTX00735 DNMT3B antibody. Left : Dnmt3b in wild-type Right : Dnmt3b/3a knockout Dilution : 1:5000 Fixation : 4% PFA/PBS for 30 min at 4 degree C
ICC/IF analysis of mouse embryonic stem cells using GTX00735 DNMT3B antibody. Left : Dnmt3b in wild-type Right : Dnmt3b/3a knockout Dilution : 1:5000 Fixation : 4% PFA/PBS for 30 min at 4 degree C
DNMT3B antibody - KO/KD, Orthogonal, Overexpression Validated
GTX00735
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityMouse
TargetDnmt3b
Overview
- SupplierGeneTex
- Product NameDNMT3B antibody - KO/KD, Orthogonal, Overexpression Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes with mouse Dnmt3b isoforms 3b1, 3b2, 3b3 and 3b6, but does not react with Dnmt3a due to its immunogen.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration~0.3 mg/ml
- ConjugateUnconjugated
- Gene ID13436
- Target nameDnmt3b
- Target descriptionDNA methyltransferase 3B
- Target synonymsDNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase MmuIIIB; DNA MTase MmuIIIB; MmuIIIB
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]
- ReactivityMouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203