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Dnmt3l Antibody - middle region (OAAB19087)

OAAB19087
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetDnmt3l
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Dnmt3l Antibody - middle region (OAAB19087)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Gene ID54427
  • Target name
    Dnmt3l
  • Target description
    DNA (cytosine-5-)-methyltransferase 3-like
  • Target synonyms
    D6Ertd14; D6Ertd14e; DNA (cytosine-5)-methyltransferase 3-like; ecat; ecat7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dnmt3l using anti-Dnmt3l antibody (A04214-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt3l antigen affinity purified polyclonal antibody (Catalog # A04214-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt3l at approximately 44 kDa. The expected band size for Dnmt3l is at 44 kDa.
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TargetDnmt3l
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