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Doublin Monoclonal Antibody

BSM-63046R
Bioss Antibodies
ApplicationsWestern Blot
Product group Antibodies
TargetDCX
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Doublin Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Recombinant
  • Conjugate
    Unconjugated
  • Gene ID1641
  • Target name
    DCX
  • Target description
    doublecortin
  • Target synonyms
    DBCN; DC; doublecortex; doublin; lissencephalin-X; LISX; lis-X; neuronal migration protein doublecortin; SCLH; XLIS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43602
  • Protein Name
    Neuronal migration protein doublecortin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11c/Itgax antigen affinity purified polyclonal antibody (Catalog # A00357-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11c/Itgax at approximately 150KD. The expected band size for CD11c/Itgax is at 150KD.
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