DYKDDDDK (FLAG) Positive Control Lysate

Biorbyt

DYKDDDDK (FLAG) Positive Control Lysate

10

ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 microl is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.

Western Blot, Other Application

Research Use Only

1 mg/ml

Unconjugated

DYKDDDDK (FLAG) Positive Control Lysate

41105330