DYKDDDDK (FLAG tag) antibody (Agarose)

Biorbyt

DYKDDDDK (FLAG tag) antibody (Agarose)

16

Anti-DYKDDDDK Affinity Gel has been tested by SDS-Page, IP, and western blot and is optimally suited for immunoprecipitation and purification of FLAG tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG is a registered trademark of Sigma-Aldrich. Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.

ImmunoPrecipitation, Western Blot

IP: 10 µL resin binds >1 µg FLAG fusion protein IP, WB

Research Use Only

Monoclonal

29E4.G7

Agarose

Mouse

IgG2a

Mouse monoclonal antibody against DYKDDDDK (FLAG tag) conjugated to Agarose.

2°C to 8°C

12352203