Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (TA383024) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 5s.
Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (TA383024) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 5s.
E2 230K (UBE2O) Rabbit Polyclonal Antibody
TA383024
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUBE2O
Overview
- SupplierOriGene
- Product NameE2 230K (UBE2O) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID63893
- Target nameUBE2O
- Target descriptionubiquitin conjugating enzyme E2 O
- Target synonyms(E3-independent) E2 ubiquitin-conjugating enzyme; E2/E3 hybrid ubiquitin-protein ligase UBE2O; E2-230K; ubiquitin carrier protein O; ubiquitin conjugating enzyme E2O; ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin-conjugating enzyme E2-230K; ubiquitin-protein ligase O
- HostRabbit
- IsotypeIgG
- Protein IDQ9C0C9
- Protein Name(E3-independent) E2 ubiquitin-conjugating enzyme
- Scientific DescriptionUBE2O Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203