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ProductsBack/E2 230K (UBE2O) Rabbit Polyclonal Antibody
Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (TA383024) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (TA383024) at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 1s.
Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (TA383024) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

E2 230K (UBE2O) Rabbit Polyclonal Antibody

TA383024
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUBE2O
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    E2 230K (UBE2O) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID63893
  • Target name
    UBE2O
  • Target description
    ubiquitin conjugating enzyme E2 O
  • Target synonyms
    E2-230K, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, ubiquitin carrier protein O, ubiquitin conjugating enzyme E2O, ubiquitin-conjugating enzyme E2 of 230 kDa, ubiquitin-conjugating enzyme E2-230K, ubiquitin-protein ligase O
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0C9
  • Protein Name
    (E3-independent) E2 ubiquitin-conjugating enzyme
  • Scientific Description
    UBE2O Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of UBE2O using anti-UBE2O antibody (A09217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2O antigen affinity purified polyclonal antibody (Catalog # A09217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2O at approximately 200 kDa. The expected band size for UBE2O is at 141 kDa.
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