Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E2F1 Polyclonal Antibody, Unconjugated (bs-23185R) at 1:500 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with E2F1 Polyclonal Antibody, Unconjugated (bs-23185R) at 1:500 overnight at 4°C, DAB staining.
E2F1 Polyclonal Antibody
BS-23185R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetE2F1
Overview
- SupplierBioss Antibodies
- Product NameE2F1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1869
- Target nameE2F1
- Target descriptionE2F transcription factor 1
- Target synonymsE2F-1; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; retinoblastoma-associated protein 1; retinoblastoma-binding protein 3; transcription factor E2F1
- HostRabbit
- IsotypeIgG
- Protein IDQ01094
- Protein NameTranscription factor E2F1
- ReactivityBovine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203