WB analysis of A431 cell extracts with/without EGF (100 ng/ml, 37oC, 30 mins) treatment using GTX00958 EGFR (phospho Tyr1068) antibody [GT1196]. Dilution : 1:1000 Loading : 25 microg
![Untreated (-) and treated (+) A431 whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1068) antibody [GT1196] (GTX00958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00958/GTX00958_4000000013_20200410_WB_treatment_EGF_w_23053121_249.webp "Untreated (-) and treated (+) A431 whole cell extracts (30 microg) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1068) antibody [GT1196] (GTX00958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of A431 cell extracts with/without EGF (100 ng/ml, 37oC, 30 mins) treatment using GTX00958 EGFR (phospho Tyr1068) antibody [GT1196]. Dilution : 1:1000 Loading : 25 microg
EGFR (phospho Tyr1068) antibody [GT1196]
GTX00958
Overview
- SupplierGeneTex
- Product NameEGFR (phospho Tyr1068) antibody [GT1196] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThe full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1068 of mature EGFR is equivalent to Tyr1092 of uncleaved EGFR.
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1196
- ConjugateUnconjugated
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostRabbit
- IsotypeIgG
- Protein IDP00533
- Protein NameEpidermal growth factor receptor
- Scientific DescriptionThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203