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ProductsBack/EGFR (phospho Tyr1148) antibody
WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF (Lane 2-5) for 15 minutes using GTX10643 EGFR (phospho Tyr1148) antibody prior incubated with, non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF (Lane 2-5) for 15 minutes using GTX10643 EGFR (phospho Tyr1148) antibody prior incubated with, non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of A431 cells stimulated with 200 ng/mL EGF (Lane 2-5) for 15 minutes using GTX10643 EGFR (phospho Tyr1148) antibody prior incubated with, non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

EGFR (phospho Tyr1148) antibody

GTX10643
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Mouse
TargetEGFR
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Overview

  • Supplier
    GeneTex
  • Product Name
    EGFR (phospho Tyr1148) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Antibody Specificity
    The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1148 of mature EGFR is equivalent to Tyr1172 of uncleaved EGFR.
  • Application Supplier Note
    ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1956
  • Target name
    EGFR
  • Target description
    epidermal growth factor receptor
  • Target synonyms
    avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00533
  • Protein Name
    Epidermal growth factor receptor
  • Scientific Description
    The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203