WB using anti-EGFR antibody shows detection of a band at ~170 kDa corresponding to human EGFR present in unstimulated (lane 1) and EGF (50 ng/ml for 15 min) stimulated (lane 2) A431 whole cell lysates (arrowhead). The primary antibody diluted to 1:1,000. Reaction occurred overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at RT.
WB using anti-EGFR antibody shows detection of a band at ~170 kDa corresponding to human EGFR present in unstimulated (lane 1) and EGF (50 ng/ml for 15 min) stimulated (lane 2) A431 whole cell lysates (arrowhead). The primary antibody diluted to 1:1,000. Reaction occurred overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at RT.
EGFR Rabbit Polyclonal Antibody
TA319215
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEGFR
Overview
- SupplierOriGene
- Product NameEGFR Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostRabbit
- IsotypeIgG
- Protein IDP00533
- Protein NameEpidermal growth factor receptor
- Scientific DescriptionRabbit polyclonal anti-EGFR antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203