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EGR2 Antibody (clone OTI1F10)

LS-C174272
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetEGR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EGR2 Antibody (clone OTI1F10)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:100), WB (1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1F10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1959
  • Target name
    EGR2
  • Target description
    early growth response 2
  • Target synonyms
    AT591; CHN1; CMT1D; CMT4E; E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; early growth response protein 2; KROX20; KROX-20, Drosophila, homolog (early growth response-2); zinc finger protein Krox-20
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EGR2 using anti-EGR2 antibody (A00921-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR2 antigen affinity purified polyclonal antibody (Catalog # A00921-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGR2 at approximately 50 kDa. The expected band size for EGR2 is at 50,45 kDa.
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Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Rat brain tissue All lanes: EGR2 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 45 kDa Observed band size: 51 kDa
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Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates probed with EGR2 Polyclonal Antibody, Unconjugated (bs-8368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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