WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37oC, 30 min) treatment after serum-starvation overnight or TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00965 eIF2 alpha (phospho Ser51) antibody [GT1203]. Dilution : 1:1000 Loading : 25microg
eIF2 alpha (phospho Ser51) antibody [GT1203] - Recombinant antibody, Orthogonal Validated
GTX00965
Overview
- SupplierGeneTex
- Product NameeIF2 alpha (phospho Ser51) antibody [GT1203] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer10
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1203
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
- ReactivityHuman, Mouse
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352203