![WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37oC, 30 min) treatment after serum-starvation overnight or TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00965 eIF2 alpha (phospho Ser51) antibody [GT1203]. Dilution : 1:1000 Loading : 25microg WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37oC, 30 min) treatment after serum-starvation overnight or TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00965 eIF2 alpha (phospho Ser51) antibody [GT1203]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00965/GTX00965_20200327_WB_1_w_23053121_538.webp)
WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37oC, 30 min) treatment after serum-starvation overnight or TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00965 eIF2 alpha (phospho Ser51) antibody [GT1203]. Dilution : 1:1000 Loading : 25microg
eIF2 alpha (phospho Ser51) antibody [GT1203]
GTX00965
Overview
- SupplierGeneTex
- Product NameeIF2 alpha (phospho Ser51) antibody [GT1203] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1203
- ConjugateUnconjugated
- Gene ID1965
- Target nameEIF2S1
- Target descriptioneukaryotic translation initiation factor 2 subunit alpha
- Target synonymsEIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- HostRabbit
- IsotypeIgG
- Protein IDP05198
- Protein NameEukaryotic translation initiation factor 2 subunit 1
- Scientific DescriptionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203