Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-eIF2 alpha (Ser51) Polyclonal Antibody, Unconjugated (bs-4842R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
eIF2 alpha subunit 1 (Ser52) Antibody
BS-4842R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
Overview
- SupplierBioss Antibodies
- Product NameeIF2 alpha subunit 1 (Ser52) Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- ReactivityBovine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP. Du L et al., 2018, Biomed Res IntRead more
- miR-211 Plays a Critical Role in Cnidium officinale Makino Extract-Induced, ROS/ER Stress-Mediated Apoptosis in U937 and U266 Cells. Cha JA et al., 2018 Mar 15, Int J Mol SciRead more
- Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment. Berger SL et al., 2018 Feb 7, NeuronRead more
- Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Li L et al., 2018 Mar 15, Toxicol LettRead more
- A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure. Yao Y et al., 2017 Jul 25, Nat CommunRead more