WB (peptide competition) analysis of recombinant eIF2B epsilon treated with lambda phosphatase (Lane 1) or GSK-3beta (Lane 2-5) using GTX24775 EIF2B epsilon (phospho Ser539) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of recombinant eIF2B epsilon treated with lambda phosphatase (Lane 1) or GSK-3beta (Lane 2-5) using GTX24775 EIF2B epsilon (phospho Ser539) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
EIF2B epsilon (phospho Ser539) antibody
GTX24775
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEIF2B5
Overview
- SupplierGeneTex
- Product NameEIF2B epsilon (phospho Ser539) antibody
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID8893
- Target nameEIF2B5
- Target descriptioneukaryotic translation initiation factor 2B subunit epsilon
- Target synonymsCACH, CLE, EIF-2B, EIF2Bepsilon, LVWM, VWM5, translation initiation factor eIF2B subunit epsilon, eIF-2B GDP-GTP exchange factor subunit epsilon, eIF2B GDP-GTP exchange factor subunit epsilon, eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, translation initiation factor eIF-2B subunit epsilon
- HostRabbit
- IsotypeIgG
- Protein IDQ13144
- Protein NameTranslation initiation factor eIF2B subunit epsilon
- Scientific DescriptionThis gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Tardif N, Salles J, Guillet C, et al. Muscle ectopic fat deposition contributes to anabolic resistance in obese sarcopenic old rats through eIF2α activation. Aging Cell. 2014,13(6):1001-11. doi: 10.1111/acel.12263Read this paper
- Mascher H, Ekblom B, Rooyackers O, et al. Enhanced rates of muscle protein synthesis and elevated mTOR signalling following endurance exercise in human subjects. Acta Physiol (Oxf). 2011,202(2):175-84. doi: 10.1111/j.1748-1716.2011.02274.xRead this paper
- Burd NA, Holwerda AM, Selby KC, et al. Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. J Physiol. 2010,588(Pt 16):3119-30. doi: 10.1113/jphysiol.2010.192856Read this paper
- Fry CS, Glynn EL, Drummond MJ, et al. Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men. J Appl Physiol (1985). 2010,108(5):1199-209. doi: 10.1152/japplphysiol.01266.2009Read this paper
- Drummond MJ, Fry CS, Glynn EL, et al. Rapamycin administration in humans blocks the contraction-induced increase in skeletal muscle protein synthesis. J Physiol. 2009,587(Pt 7):1535-46. doi: 10.1113/jphysiol.2008.163816Read this paper
- Moore DR, Robinson MJ, Fry JL, et al. Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr. 2009,89(1):161-8. doi: 10.3945/ajcn.2008.26401Read this paper
- Glover EI, Oates BR, Tang JE, et al. Resistance exercise decreases eIF2Bepsilon phosphorylation and potentiates the feeding-induced stimulation of p70S6K1 and rpS6 in young men. Am J Physiol Regul Integr Comp Physiol. 2008,295(2):R604-10. doi: 10.1152/ajpregu.00097.2008Read this paper
Datasheet
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