Western blot using affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
Western blot using affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
EIF3F Rabbit Polyclonal Antibody
TA319432
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Chicken, Human, Mouse, Primate, Rat
TargetEIF3F
Overview
- SupplierOriGene
- Product NameEIF3F Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID8665
- Target nameEIF3F
- Target descriptioneukaryotic translation initiation factor 3 subunit F
- Target synonymsdeubiquitinating enzyme eIF3f; eIF-3-epsilon; eIF3-epsilon; eIF3-p47; EIF3S5; eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; MRT67
- HostRabbit
- IsotypeIgG
- Protein IDO00303
- Protein NameEukaryotic translation initiation factor 3 subunit F
- Scientific DescriptionRabbit polyclonal anti-EIF3F antibody
- ReactivityBovine, Chicken, Human, Mouse, Primate, Rat
- Storage Instruction-20°C
- UNSPSC12352203