
ICC/IF analysis of methanol-fixed MCF cells using GTX50268 eIF4E (phospho Ser209) antibody.
eIF4E (phospho Ser209) antibody
GTX50268
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mammals, Mouse
TargetEIF4E
Overview
- SupplierGeneTex
- Product NameeIF4E (phospho Ser209) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1977
- Target nameEIF4E
- Target descriptioneukaryotic translation initiation factor 4E
- Target synonymsAUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP06730
- Protein NameEukaryotic translation initiation factor 4E
- Scientific DescriptionThe protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5 end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
- ReactivityHuman, Mammals, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- The squirrel with the lagging eIF2: Global suppression of protein synthesis during torpor. Logan SM et al., 2019 Jan, Comp Biochem Physiol A Mol Integr PhysiolRead more
- delta-Tocotrienol oxazine derivative antagonizes mammary tumor cell compensatory response to CoCl2-induced hypoxia. Ananthula S et al., 2014, Biomed Res IntRead more