ELN / Elastin Antibody

LifeSpan BioSciences

ELN / Elastin Antibody

14

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Human Elastin 100%, human Collagen Type I, II, III, IV, V <0,1%; human Fibronectin <0,1%.. ELISA (1:200), IF (1:80), IHC-P (1:500 - 1:1000), RIA After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Human Elastin 100%, human Collagen Type I, II, III, IV, V <0,1%; human Fibronectin <0,1%.

ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay

Research Use Only

Polyclonal

Unconjugated

...

ELN

elastin

ADCL1; elastin; SVAS; tropoelastin; WBS; WS

Guinea Pig

Human

-20°C

12352203