EPCAM Antibody (Cytoplasmic Domain, clone EGP40/1110, Azide-free, BSA-free)

LifeSpan BioSciences

EPCAM Antibody (Cytoplasmic Domain, clone EGP40/1110, Azide-free, BSA-free)

23

Human TACSTD1 / EPCAM

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Optimal dilution of the EpCAM antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.. IF (1 - 2 µg/ml), IHC, IHC-P (0.5 - 1 µg/ml), WB (0.5 - 1 µg/ml) The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Optimal dilution of the EpCAM antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

EGP40/1110

1 mg/ml

Unconjugated

...

EPCAM

epithelial cell adhesion molecule

adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP314; EGP40; epithelial cell adhesion molecule; epithelial glycoprotein 314; ESA; HNPCC8; human epithelial glycoprotein-2; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1

Mouse

IgG2b

Human, Mouse, Rat

2°C to 8°C

12352203