More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/EpCAM/CD326

EpCAM/CD326

MUB2061P
Nordic-MUbio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEPCAM
0.1 mg
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    EpCAM/CD326
  • Delivery Days Customer
    7
  • Antibody Specificity
    The antibody HEA125 is directed against human EpCAM.
  • Application Supplier Note
    The HEA125 antibody is suitable for the detection of human EpCAM by Western blotting (under reducing and non-reducing conditions), flow cytometry and immunocytochemistry. The antibody is also suitable for the detection of human EpCAM by immunohistochemistry on frozen and paraffin embedded tissues. For the latter application, pre-treatment with pronase 0.1% or unmasking fluid C (Art. No. DE000) is recommended. Optimal antibody dilutions for the different applications should be determined by titration. For immmunohistochemistry a dilution of 1:100 to 1:500 is recommended. The monoclonal antibody HEA125 shows a broad reactivity pattern with most human epithelial cells and epithelium derived tumours. It doesnt react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunofluorescence;Immunofluorescence;ELISA;Flow Cytometry;Immunohistochemistry (frozen & paraffin);Immunohistochemistry (frozen & paraffin);Immunohistochemistry (frozen & paraffin);Immunohistochemistry (frozen & paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HEA125
  • Concentration
    1 mg/ml
  • Gene ID4072
  • Target name
    EPCAM
  • Target description
    epithelial cell adhesion molecule
  • Target synonyms
    adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; DIAR5; EGP-2; EGP314; EGP40; epithelial cell adhesion molecule; epithelial glycoprotein 314; ESA; HNPCC8; human epithelial glycoprotein-2; KS1/4; KSA; M4S1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16422
  • Protein Name
    Epithelial cell adhesion molecule
  • Shelf life instruction
    For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline
  • Source
    HEA125 is a mouse monoclonal IgG1 antibody derived by fusion of Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse cells immunized with the HT-29 colon carcinoma cell line.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Viable HT-29 human colon carcinoma cell line
  • UNSPSC
    12352203