EphA8 antibody

GeneTex

EphA8 antibody

9

WB: 0.1-0.2 microg/ml. ELISA: 0.5-1.0 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

Epha8

Eph receptor A8

AW047546; Eek; Eph; EPH- and ELK-related kinase; ephrin type-A receptor 8; Ephrin type-A receptor 8 precursor (Tyrosine-protein kinase receptor EEK) (EPH-and ELK-related kinase); Hek3; mKIAA1459; tyrosine-protein kinase receptor EEK

Goat

IgG

Ephrin type-A receptor 8

EphA8, also known as Eek and Hek3, is a member of the Eph receptor family, which binds members of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region is a juxtamembrane region with two tyrosines, the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the C-terminus, the latter including one conserved tyrosine. The calculated molecular mass of the reduced mouse EphA8/Fc monomer is 84.2 kDa, but as a result of glycosylation, recombinant EphB8/Fc migrates as an approximately 100-120 kDa protein under reducing conditions in SDS-PAGE. Ligand recognition and binding leads to activation of the intrinsic kinase activity. EphA8 binds to Ephrin-A2, Ephrin-A3, and Ephrin-A5. Full length human and rat homolgs have not been successfully cloned. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation.The ephrin ligands and eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

Mouse

-20°C or -80°C,2°C to 8°C

12352203