Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen receptor alpha) polyclonal Antibody, Unconjugated (bs-0254R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen receptor alpha) polyclonal Antibody, Unconjugated (bs-0254R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
ER-Alpha Polyclonal Antibody
BS-0254R
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetESR1
Overview
- SupplierBioss Antibodies
- Product NameER-Alpha Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, Western Blot, ELISA
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2099
- Target nameESR1
- Target descriptionestrogen receptor 1
- Target synonymsE2 receptor alpha; ER; Era; ER-alpha; ESR; ESRA; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha
- HostRabbit
- IsotypeIgG
- Protein IDP03372
- Protein NameEstrogen receptor
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203