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WB analysis of human ovary tissue lysate using GTX17208 ERAP2 antibody. Working concentration : 1 microg/ml
WB analysis of human ovary tissue lysate using GTX17208 ERAP2 antibody. Working concentration : 1 microg/ml
WB analysis of human ovary tissue lysate using GTX17208 ERAP2 antibody. Working concentration : 1 microg/ml

ERAP2 antibody

GTX17208
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetERAP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ERAP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64167
  • Target name
    ERAP2
  • Target description
    endoplasmic reticulum aminopeptidase 2
  • Target synonyms
    L-RAP, LRAP, endoplasmic reticulum aminopeptidase 2, leukocyte-derived arginine aminopeptidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P179
  • Protein Name
    Endoplasmic reticulum aminopeptidase 2
  • Scientific Description
    Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 (MIM 606832) and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2; MIM 190470) (Tanioka et al., 2003 [PubMed 12799365]).[supplied by OMIM, Mar 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERAP2 using anti-ERAP2 antibody (A04269-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERAP2 antigen affinity purified polyclonal antibody (Catalog # A04269-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERAP2 at approximately 110 kDa. The expected band size for ERAP2 is at 110 kDa.
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