CoIP and WB of a human cell lysate using anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-b1 for 15 at 100 ng/ml and without (-) the addition of Heregulin-b1. The combination of IP and WBting was performing using 5 microl of the anti-HER3 antibody for IP followed by WB detection using an anti-phosphotyrosine antibody. Visualization occurred using an ECL system. Film exposure approximately 1.