erbB4 activation Serum starved MDA-MB468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB4 - 4C6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF; lane 6: 2h EGF lane 7: 4h EGF; lane 8: 8h EGF
erbB4 activation Serum starved MDA-MB468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB4 - 4C6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF; lane 6: 2h EGF lane 7: 4h EGF; lane 8: 8h EGF
ErbB 4 (ERBB4) pTyr1242 (incl. pos. Control) Mouse Monoclonal Antibody [Clone ID: 4C6]
AM00057PU-N
Overview
- SupplierOriGene
- Product NameErbB 4 (ERBB4) pTyr1242 (incl. pos. Control) Mouse Monoclonal Antibody [Clone ID: 4C6]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4C6
- Gene ID2066
- Target nameERBB4
- Target descriptionerb-b2 receptor tyrosine kinase 4
- Target synonymsALS19; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; HER4; human epidermal growth factor receptor 4; p180erbB4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
- HostMouse
- IsotypeIgG1
- Scientific DescriptionErbB 4 (ERBB4) pTyr1242 (incl. pos. Control) mouse monoclonal antibody, clone 4C6, Purified
- ReactivityHuman
- UNSPSC12352203