ERBB2 / HER2 Antibody (Cytoplasmic Domain)

LifeSpan BioSciences

ERBB2 / HER2 Antibody (Cytoplasmic Domain)

23

Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 ug/mg protein lysate. Western Blot: 1-2 ug/ml for 2hr at RT. Immunohistochemistry: 2-4 ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma.. IHC (2 - 4 µg/ml), IP, WB (1 - 2 µg/ml) Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 ug/mg protein lysate. Western Blot: 1-2 ug/ml for 2hr at RT. Immunohistochemistry: 2-4 ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

Research Use Only

Monoclonal

Unconjugated

...

ERBB2

erb-b2 receptor tyrosine kinase 2

CD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2

Mouse

IgG1

-20°C,2°C to 8°C

12352203