Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated (bsm-51655M) at 1:25 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated (bsm-51655M) at 1:25 overnight at 4°C, DAB staining.
ERK1 Monoclonal Antibody
BSM-51655M
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetMAPK3
Overview
- SupplierBioss Antibodies
- Product NameERK1 Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(WB=1:500-2000，IHC-P=1:25，ICC=1:25，IF=1:25，), IHC-P(1:200-400), IF()
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDH8T6
- Concentration0.5 ug/ml
- ConjugateUnconjugated
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3
- HostMouse
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- ReactivityBovine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203