Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4°C, DAB staining.
ERK1/2 Monoclonal Antibody
BSM-33232M
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK3
Overview
- SupplierBioss Antibodies
- Product NameERK1/2 Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierIHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200), IF()
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8C2
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3
- HostMouse
- IsotypeIgG
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203