Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (TA378294) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (TA378294) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
ERK2 (MAPK1) Rabbit Polyclonal Antibody
TA378294
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK1
Overview
- SupplierOriGene
- Product NameERK2 (MAPK1) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5594
- Target nameMAPK1
- Target descriptionmitogen-activated protein kinase 1
- Target synonymsERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; NS13; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
- HostRabbit
- IsotypeIgG
- Protein IDP28482
- Protein NameMitogen-activated protein kinase 1
- Scientific DescriptionPhospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203