Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (TA378295) at 1:1000 dilution or ERK1 / ERK2 antibody (A16686).Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 10s.
Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (TA378295) at 1:1000 dilution or ERK1 / ERK2 antibody (A16686).Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 10s.
ERK2 (MAPK1) Rabbit Polyclonal Antibody
TA378295
Overview
- SupplierOriGene
- Product NameERK2 (MAPK1) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5594
- Target nameMAPK1
- Target descriptionmitogen-activated protein kinase 1
- Target synonymsERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; NS13; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse
- UNSPSC12352203