Fc (IgG1): FcRn Inhibitor Screening TR-FRET Assay Kit

BPS Bioscience

Fc (IgG1): FcRn Inhibitor Screening TR-FRET Assay Kit

7

Neutralisation/Blocking

Research Use Only

The Fc (IgG1): FcRn Inhibitor Screening TR-FRET Assay Kit is designed for screening and profiling of neutralizing antibodies or inhibitors of the interaction between Fc (IgG1) and human FcRn (Neonatal Fc receptor for IgG) using TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer). This kit requires no time-consuming washing steps and comes in a convenient 384-well format, with purified Biotinylated-FcRn complex (Fc receptor amino acids 24-297 and B2M amino acids 21-119) and europium-labeled (Eu) Fc (IgG1) protein (amino acids 100-330), Dye-labeled Acceptor, and assay buffer for 400 reactions. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) Fc (IgG1), dye-labeled acceptor, biotin-labeled FcRn, and an inhibitor is incubated for one hour. Then, the fluorescence signal is measured at 620 nm and 665 nm using the TR-FRET module of a fluorescent microplate reader. When FcRn binds to Fc (IgG1), energy transfer occurs between the europium donor and the dye-labeled acceptor, producing a fluorescent signal. Inhibitors that block this interaction prevent energy transfer, reducing the fluorescent signal.

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